Alicyclobacilli are aerobic, acidophilic and thermophilic, spore-forming bacteria that occur naturally in soil. They can enter the production process via spores on the surface of windfall fruit. There, they can survive the pasteurisation process and continue to grow vegetatively under optimal conditions. The appearance of the product itself remains immaculate – there is no gas formation or discolouration.
Some Alicyclobacillus strains can produce guaiacol, which causes a strong off-flavour in juices and beverages containing fruit juice. This flavour has a chemical smell similar comparable to disinfectant. According to the latest scientific expertise, A. acidoterrestris, A. acidiphilus und A. herbarius have the potential to produce off-flavours.
Further Alicyclobacillus strains such as A. acidocaldarius and A. pomorum, which cannot produce off-flavours in beverages, therefore have less or no potential for beverage spoiling.
As even low quantities of bacteria can result in the products being spoiled, it is especially important to detect not only Alicyclobacilli in general, but also to identify the guaiacol-forming species without any doubt.
Consisting of ready-to-use BAT Culture Media and the enzymatic Guaiacol Detection Kit, the comprehensive solution allows guaiacol-producing alicyclobacilli to be detected quickly and reliably. This simple detection complies with IFU (International Fruit Juice Union) Method No.12 “Method on Detection of taint producing Alicyclobacillus in Fruit Juices”.
BAT Culture Media and the Guaiacol Detection Kit are suitable for beverages containing juice with a pH of less than 4.5, such as<br/>
Reliable and sensitive enzymatic detection of guaiacol-forming alicyclobacilli
When guaiacol-forming bacteria are present, the transparent to slightly yellow guaiacol is transformed into the clearly-visible, brownish complex tetraguaiacol with the enzyme peroxidase from the Detection Kit.
|Product name||Order number||Description||Content|
|BAT-A||2.04719.782||Selective agar for isolation|
Basic nutrient medium
for membrane filtration and
|9 x 250 ml|
|BAT-B||2.04717.782||Selective liquid medium|
for fast trace detection
Sample enrichment – especially
in cloudy beverages
and beverage raw materials
|9 x 250 ml|
|Guaiacol Detection Kit||2.04737.991||Enzymatic kit for the|
detection of Alicyclobacilli
|Dematech Pty Ltd.||13 Michigan Street|
|email@example.com||+27-(021) 385 04 83|
|Australia||Directus Australia||96 Woolwich Rd|
|firstname.lastname@example.org||+61 2 9844 2100|
|China||Shanghai Sunny Biological Science|
and Technology Co.Ltd
|email@example.com||+86 021 55884753|
+86 135 24671568
|New Zealand||Directus New Zealand||Suite 2, Level 1, Heards|
168 Parnell Road
|firstname.lastname@example.org||+64 9 309 7783|
|USA||Weber Scientific||2732 Kuser Road|
Hamilton, NJ, 08691
|email@example.com||+1 800 328 8378|
|rest of world||Döhler GmbH|
*excl.: Libya, Sudan, Morocco, Mauritania, Western Sahara, Tunisia, Egypt, Algeria