NBB®-B-Am ready-to-use broth for the detection

of biofilms as part of hygiene monitoring in the beverage industry.

Product description

Biofilms are accumulations of various microorganisms on living and non-living surfaces. Biofilms develop in four characteristic phases: 1. transportation of the microorganisms to the surface, 2. reversible attachment to the surface, 3. irreversible, permanent accumulation and fixation, 4. subsequent colonisation and complex association of different microorganisms.

Mucous-forming acetic acid bacteria and other ubiquitous mucous-formers such as Enterobacter cloacae, Klebsiella pneumoniae, Cryptococcus spp., Rhodotorula spp. and others play a particular role in irreversible accumulation. The mucous capsules of these organisms offer outstanding protection against drying, heat and disinfectants. They thus also promote colonisation by other microorganisms, which also benefit from the protection of the mucous capsules. Because acetic acid bacteria and other mucous-formers consume the oxygen in the coating very quickly, an ideal low-oxygen or even oxygen-free microenvironment is created for microaerophilic and anaerobic beverage-spoiling microorganisms. Strictly anaerobic beer-spoiling microorganisms such as Pectinatus and Megasphera can grow very well here. Because lactic acid bacteria often colonise these areas, there are usually relevant amounts of lactic acid present, which is metabolised by Pectinatus and Megasphera. The autolysis by the now more mature microorganisms causes a use of the pH value, which supports the growth of dangerous beer-spoiling microorganisms.

Similarly, microorganisms such as yeasts and beverage-spoiling Leuconostoc and Lactobacillus species can be enriched in the facilities of non-alcoholic beverage producers. At mineral spring companies, coliform bacteria, Escherichia coli and faecal streptococci have been detected in mucous layers.

The biofilms in phase 4, complex association, are persistent biofilms that indicate insufficient hygiene and result in an increased risk of secondary contamination in the end products. 

Weak point and trend analyses during beverage production are essential elements of an effective biological quality control programme.

The ready to use NBB®-B-Am Broth allows weak points in your production and filling facility to be monitored through the simple use of swab samples.  Swab samples with sterile swabs are taken at critical points of the filling line and incubated directly in ready-to-use NBB® B-Am Broth. The indicator colour changes from red to yellow, making visual analysis even simpler and allowing clear and fast detection of biofilm indicator microorganisms.  

The biological condition of a production facility is immaculate if an average of less than 20% of the results found with NBB®-B-Am are positive over several weeks. An increased or increasing proportion of positive results indicate persistent biofilms, in which relevant beverage or beer-spoiling microorganisms or other problematic bacteria can settle and adapt gradually over time.

NBB®-B-Am offers you the following user benefits: 

  • Simple hygiene monitoring for weak point analyses and trending
  • Sampling by means of smear swabs at critical points throughout the production process
  • Direct use of liquid medium - ready-to-use in sterile tubes or sterile bottle for individual portioning
  • Simple, visual screening by an indicator change from red to yellow

Order information

Product nameOrder numberDescriptionContent
NBB®-B-Am2.04706.782 Nutrient medium for qualitative detection
of indicator microorganisms
after sampling
with sterile swabs
Use of microbiological facility monitoring
9 x 250 ml
in tube
2.04706.646 Culture medium for qualitative detection
of indicator microorganisms
after sampling with sterile swabs
For microbiological facility monitoring
using an easy evaluation in glass tubes
with screw closure for direct application
20 x 10 ml
Smear swabs2.04725.444Sterile swabs,
cotton swabs on wooden sticks,
individually packaged,
without tubes
Smear samples
100 pc. (bag)

Basic information for NBB®-B-Am

pH valueIncubation T [°C]Duration (days)
5.75 +/- 0.1527Max. 3

Storage and shipping information for NBB®-B-Am

Storage temperature T [°C]Shelf life (months)

Packaging information for NBB®-B-Am

9 x 250 mlGlass bottles in cardboard box

Basic information for NBB®-B-Am in tubes

pH valueIncubation T [°C]Duration (days)
5.75 +/- 0.1527Max. 3

Storage and shipping information for NBB®-B-Am in tubes

Storage temperature T [°C]Shelf life (months)

Packaging information for NBB®-B-Am in tubes

20 x 10 mlGlass tubes with screw closure,
polystyrene box

Storage and shipping information for smear swabs

Storage temperature T [°C]Shelf life (months)
20-25 °CSee information on product

Packaging information for smear swabs

100 pc.Individually wrapped,
in bag

Please contact our distributors or Döhler directly.



Dematech Pty Ltd.13 Michigan Street
Airport Industria
Capetown 7490
South Africa
info@dematechsa.com+27-(021) 385 04 83
AustraliaDirectus Australia96 Woolwich Rd
Hunters Hill
NSW, 2110
sales@directus.com.au+61 2 9844 2100
ChinaShanghai Sunny Biological Science
and Technology Co.Ltd

Room 1706
No.1619 Dalian road
Hongkou District
Zip: 200086

yanhqx@sina.com+86 021 55884753
+86 135 24671568
New ZealandDirectus New ZealandSuite 2, Level 1, Heards
168 Parnell Road
Auckland, 1052
New Zealand
sales@directus.co.nz+64 9 309 7783
USAWeber Scientific2732 Kuser Road
Hamilton, NJ, 08691
info@weberscientific.com+1 800 328 8378
rest of worldDöhler GmbH

Riedstraße 7-9
64295 Darmstadt

dmd@doehler.com+49 (0)6151/306-0

*excl.: Libya, Sudan, Morocco, Mauritania, Western Sahara, Tunisia, Egypt, Algeria

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