Biofilms are accumulations of various microorganisms on living and non-living surfaces. Biofilms develop in four characteristic phases: 1. transportation of the microorganisms to the surface, 2. reversible attachment to the surface, 3. irreversible, permanent accumulation and fixation, 4. subsequent colonisation and complex association of different microorganisms.
Mucous-forming acetic acid bacteria and other ubiquitous mucous-formers such as Enterobacter cloacae, Klebsiella pneumoniae, Cryptococcus spp., Rhodotorula spp. and others play a particular role in irreversible accumulation. The mucous capsules of these organisms offer outstanding protection against drying, heat and disinfectants. They thus also promote colonisation by other microorganisms, which also benefit from the protection of the mucous capsules. Because acetic acid bacteria and other mucous-formers consume the oxygen in the coating very quickly, an ideal low-oxygen or even oxygen-free microenvironment is created for microaerophilic and anaerobic beverage-spoiling microorganisms. Strictly anaerobic beer-spoiling microorganisms such as Pectinatus and Megasphera can grow very well here. Because lactic acid bacteria often colonise these areas, there are usually relevant amounts of lactic acid present, which is metabolised by Pectinatus and Megasphera. The autolysis by the now more mature microorganisms causes a use of the pH value, which supports the growth of dangerous beer-spoiling microorganisms.
Similarly, microorganisms such as yeasts and beverage-spoiling Leuconostoc and Lactobacillus species can be enriched in the facilities of non-alcoholic beverage producers. At mineral spring companies, coliform bacteria, Escherichia coli and faecal streptococci have been detected in mucous layers.
The biofilms in phase 4, complex association, are persistent biofilms that indicate insufficient hygiene and result in an increased risk of secondary contamination in the end products.
Weak point and trend analyses during beverage production are essential elements of an effective biological quality control programme.
The ready to use NBB®-B-Am Broth allows weak points in your production and filling facility to be monitored through the simple use of swab samples. Swab samples with sterile swabs are taken at critical points of the filling line and incubated directly in ready-to-use NBB® B-Am Broth. The indicator colour changes from red to yellow, making visual analysis even simpler and allowing clear and fast detection of biofilm indicator microorganisms.
The biological condition of a production facility is immaculate if an average of less than 20% of the results found with NBB®-B-Am are positive over several weeks. An increased or increasing proportion of positive results indicate persistent biofilms, in which relevant beverage or beer-spoiling microorganisms or other problematic bacteria can settle and adapt gradually over time.
NBB®-B-Am offers you the following user benefits:
|Product name||Order number||Description||Content|
|NBB®-B-Am||2.04706.782|| Nutrient medium for qualitative detection|
of indicator microorganisms
with sterile swabs
Use of microbiological facility monitoring
|9 x 250 ml|
|2.04706.646|| Culture medium for qualitative detection|
of indicator microorganisms
after sampling with sterile swabs
For microbiological facility monitoring
using an easy evaluation in glass tubes
with screw closure for direct application
|20 x 10 ml|
|Smear swabs||2.04725.444||Sterile swabs,|
cotton swabs on wooden sticks,
|100 pc. (bag)|
|Dematech Pty Ltd.||13 Michigan Street|
|email@example.com||+27-(021) 385 04 83|
|Australia||Directus Australia||96 Woolwich Rd|
|firstname.lastname@example.org||+61 2 9844 2100|
|China||Shanghai Sunny Biological Science|
and Technology Co.Ltd
|email@example.com||+86 021 55884753|
+86 135 24671568
|New Zealand||Directus New Zealand||Suite 2, Level 1, Heards|
168 Parnell Road
|firstname.lastname@example.org||+64 9 309 7783|
|USA||Weber Scientific||2732 Kuser Road|
Hamilton, NJ, 08691
|email@example.com||+1 800 328 8378|
|rest of world||Döhler GmbH|
*excl.: Libya, Sudan, Morocco, Mauritania, Western Sahara, Tunisia, Egypt, Algeria