NBB®-B-Am Broth

Detection of biofilm indicator microorganisms

Biofilms are accumulations of various microorganisms which can develop into complex colonies over time. Starting out with ubiquitous bacteria, such as acetic acid bacteria and mucous-forming microorganisms which commonly form on substrate residue in inaccessible sections on the filling line, a biofilm gradually establishes the ideal conditions for beer and beverage-spoiling microorganisms; even for strictly anaerobic bacteria such as Pectinatus and Megasphaera. If the biofilm breaks apart, the bacteria are spread throughout the environment by aerosols which may lead to the contamination of individual bottles. This is called scatter contamination or secondary contamination.

The ready-to-use NBB®-B-Am Broth allows weak points in your production and filling to be monitored continuously through the detection of indicator bacteria for biofilms using smear samples. Sampling with sterile swabs is performed at defined critical points 1-2 times a week. The samples are then incubated in NBB®-B-Am Broth and analysed for indicators such as acetic acid bacteria or mucous-forming microorganisms, etc. A positive test result is indicated when the colour changes from red to yellow. The number of positive results is then documented as a percentage.

The biological condition of a non-sterile production facility is regarded as non-critical if the number of positive results remain below the safety limit of 20% - 30%. Percentages above the limit values, or increasing numbers of positive results over a period of several weeks indicate the development of persistent biofilms. The production line must be thoroughly cleaned and disinfected to return to the non-critical range and prevent any risk of secondary contamination.

Benefits of product

Application benefits

- Ready-to-use nutrient media - Easy handling
- Available as single test - Continuous hygiene monitoring
- Functionality tested - Additional analyses

Order information


Article no.


NBB®-B-Am (Broth)



9 x 250 ml in glass bottles

20 x 10 ml in glass tubes

Further product recommendations

Please contact the following distributors or Döhler directly.

Country Name Adress Email Phone
China Shanghai Sunny Biological Science
and Technology Co.Ltd
Room 1706, No.1619 Dalian road,
Hongkou District,
yhq@shennei.com +86 021 65190298 8016

Avantor SEA

18 Gul Drive
Singapore 629468
tharachaya.woraraktiwongs@avantorinc.com +66 95 5626963
Mongolia Monbiopharm Co. Ltd. Sukhbaatar District - 4 khoroo 1-69
First 40000
info@monbiopharm.mn +976 11 330234
Brazil Prozyn Indústria e Comércio Ltda Rua Dr. Paulo Leite de Oliveira, 199
São Paulo/SP
flavio.melo@prozyn.com.br +55 11 3732 0000
Brazil Merse R. Domingos Cazotti, 284
Jd. Santa Genebra - Campinas/SP 
valkiria@merse.com.br +55 193733 3800
USA Weber Scientific 2732 Kuser Road
Hamilton, NJ, 08691
swilson@weberscientific.com +1 609 249 1408
USA White Labs 9495 Candida St
San Diego, CA 92126
nparker@whitelabs.com +1 858 527 7358
USA BSG 9650 East 40th Ave
Denver, CO 80238
rnelson@bsgcraftbrewing.com +1 303 574 8955
Canada ProSource Scientific 5370 Canotek Road Unit 11
Ontario K1J 9E7
duane@psscientific.com +1 866 939 9959
France Laboratories Humeau ZA Gesvrine - 4 rue Kepler BP 4125
44241 La Chapelle-sur-Erdre Cedex
m.minier@humeau.com +33 2 40 93 53 53
France Brewpark sas Rue des Colinettes
ZI Mardeuil, 51530 Mardeuil
michael.gelender@brewpark.com +33 3 26 55 82 89
Italy Sacco S.r.l. Via A. Manzoni 29/A
22071 Cadorago
p.berardinetti@saccosrl.it +39 031 8866607
Latvia SnapTest.LV SIA Valmieras iela 31B
Rīga, LV-1009
gints@snaptest.lv +371 26 53 47 45
United Kingdom Trafalgar Scientific Ltd 190 Waterside Rd, Leicester LE5 1QZ asmith@trafalgarscientific.co.uk  +44 116 287 9460
Netherlands SBI Europe Kraaiondonk 44
5428 NZ Venhorst
The Netherlands
pvanrooij@sbi4beer.com +31 413 783 880
all European countries VWR International Hilpertstraße 20a
64295 Darmstadt
info.de@vwr.com +49 800 702 00 07
all other countries Döhler GmbH Riedstraße 7-9
64295 Darmstadt
dmd@doehler.com +49 (0)6151 3060

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