NBB®-B-Am Broth

Detection of biofilm indicator microorganisms

Biofilms are accumulations of various microorganisms which can develop into complex colonies over time. Starting out with ubiquitous bacteria, such as acetic acid bacteria and mucous-forming microorganisms which commonly form on substrate residue in inaccessible sections on the filling line, a biofilm gradually establishes the ideal conditions for beer and beverage-spoiling microorganisms; even for strictly anaerobic bacteria such as Pectinatus and Megasphaera. If the biofilm breaks apart, the bacteria are spread throughout the environment by aerosols which may lead to the contamination of individual bottles. This is called scatter contamination or secondary contamination.

The ready-to-use NBB®-B-Am Broth allows weak points in your production and filling to be monitored continuously through the detection of indicator bacteria for biofilms using smear samples. Sampling with sterile swabs is performed at defined critical points 1-2 times a week. The samples are then incubated in NBB®-B-Am Broth and analysed for indicators such as acetic acid bacteria or mucous-forming microorganisms, etc. A positive test result is indicated when the colour changes from red to yellow. The number of positive results is then documented as a percentage.

The biological condition of a non-sterile production facility is regarded as non-critical if the number of positive results remain below the safety limit of 20% - 30%. Percentages above the limit values, or increasing numbers of positive results over a period of several weeks indicate the development of persistent biofilms. The production line must be thoroughly cleaned and disinfected to return to the non-critical range and prevent any risk of secondary contamination.

Benefits of product Application benefits
- Ready-to-use nutrient media - Easy handling
- Available as single test - Continuous hygiene monitoring
- Functionality tested - Additional analyses

Order information

Product

Article no. Packaging
NBB®-B-Am (Broth)

204.706.782

204.706.646

9 x 250 ml in glass bottles

20 x 10 ml in glass tubes

Further product recommendations

Please contact the following distributors or Döhler directly.

CountryNameAdressEmailPhone
ChinaShanghai Sunny Biological Science
and Technology Co.Ltd
Room 1706, No.1619 Dalian road,
Hongkou District,
Shanghai
yhq@shennei.com+86 021 65190298 8016
Singapore

Avantor SEA

18 Gul Drive
Singapore 629468
tharachaya.woraraktiwongs@avantorinc.com+66 95 5626963
MongoliaMonbiopharm Co. Ltd.Sukhbaatar District - 4 khoroo 1-69
First 40000
Ulanbaatar
info@monbiopharm.mn+976 11 330234
BrazilProzyn Indústria e Comércio LtdaRua Dr. Paulo Leite de Oliveira, 199
São Paulo/SP
flavio.melo@prozyn.com.br+55 11 3732 0000
BrazilMerseR. Domingos Cazotti, 284
Jd. Santa Genebra - Campinas/SP 
valkiria@merse.com.br+55 193733 3800
USAWeber Scientific2732 Kuser Road
Hamilton, NJ, 08691
swilson@weberscientific.com+1 609 249 1408
USAWhite Labs9495 Candida St
San Diego, CA 92126
nparker@whitelabs.com+1 858 527 7358
USABSG9650 East 40th Ave
Denver, CO 80238
rnelson@bsgcraftbrewing.com+1 303 574 8955
CanadaProSource Scientific5370 Canotek Road Unit 11
Ottawa
Ontario K1J 9E7
duane@psscientific.com+1 866 939 9959
FranceLaboratories HumeauZA Gesvrine - 4 rue Kepler BP 4125
44241 La Chapelle-sur-Erdre Cedex
m.minier@humeau.com+33 2 40 93 53 53
FranceBrewpark sasRue des Colinettes
ZI Mardeuil, 51530 Mardeuil
michael.gelender@brewpark.com+33 3 26 55 82 89
ItalySacco S.r.l.Via A. Manzoni 29/A
22071 Cadorago
p.berardinetti@saccosrl.it+39 031 8866607
LatviaSnapTest.LV SIAValmieras iela 31B
Rīga, LV-1009
gints@snaptest.lv+371 26 53 47 45
United KingdomTrafalgar Scientific Ltd190 Waterside Rd, Leicester LE5 1QZasmith@trafalgarscientific.co.uk +44 116 287 9460
NetherlandsSBI EuropeKraaiondonk 44
5428 NZ Venhorst
The Netherlands
pvanrooij@sbi4beer.com+31 413 783 880
all European countriesVWR InternationalHilpertstraße 20a
64295 Darmstadt
Germany
info.de@vwr.com+49 800 702 00 07
all other countriesDöhler GmbHRiedstraße 7-9
64295 Darmstadt
Germany
dmd@doehler.com+49 (0)6151 3060

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