NBB®-B-Am ready-to-use broth for the detection

of biofilms as part of hygiene monitoring in the beverage industry.

Product description

Biofilms are accumulations of various microorganisms on living and non-living surfaces. Biofilms develop in four characteristic phases: 1. transportation of the microorganisms to the surface, 2. reversible attachment to the surface, 3. irreversible, permanent accumulation and fixation, 4. subsequent colonisation and complex association of different microorganisms.

Mucous-forming acetic acid bacteria and other ubiquitous mucous-formers such as Enterobacter cloacae, Klebsiella pneumoniae, Cryptococcus spp., Rhodotorula spp. and others play a particular role in irreversible accumulation. The mucous capsules of these organisms offer outstanding protection against drying, heat and disinfectants. They thus also promote colonisation by other microorganisms, which also benefit from the protection of the mucous capsules. Because acetic acid bacteria and other mucous-formers consume the oxygen in the coating very quickly, an ideal low-oxygen or even oxygen-free microenvironment is created for microaerophilic and anaerobic beverage-spoiling microorganisms. Strictly anaerobic beer-spoiling microorganisms such as Pectinatus and Megasphera can grow very well here. Because lactic acid bacteria often colonise these areas, there are usually relevant amounts of lactic acid present, which is metabolised by Pectinatus and Megasphera. The autolysis by the now more mature microorganisms causes a use of the pH value, which supports the growth of dangerous beer-spoiling microorganisms.

Similarly, microorganisms such as yeasts and beverage-spoiling Leuconostoc and Lactobacillus species can be enriched in the facilities of non-alcoholic beverage producers. At mineral spring companies, coliform bacteria, Escherichia coli and faecal streptococci have been detected in mucous layers.

The biofilms in phase 4, complex association, are persistent biofilms that indicate insufficient hygiene and result in an increased risk of secondary contamination in the end products. 

Weak point and trend analyses during beverage production are essential elements of an effective biological quality control programme.

The ready to use NBB®-B-Am Broth allows weak points in your production and filling facility to be monitored through the simple use of swab samples.  Swab samples with sterile swabs are taken at critical points of the filling line and incubated directly in ready-to-use NBB® B-Am Broth. The indicator colour changes from red to yellow, making visual analysis even simpler and allowing clear and fast detection of biofilm indicator microorganisms.  

The biological condition of a production facility is immaculate if an average of less than 20% of the results found with NBB®-B-Am are positive over several weeks. An increased or increasing proportion of positive results indicate persistent biofilms, in which relevant beverage or beer-spoiling microorganisms or other problematic bacteria can settle and adapt gradually over time.

NBB®-B-Am offers you the following user benefits: 

  • Simple hygiene monitoring for weak point analyses and trending
  • Sampling by means of smear swabs at critical points throughout the production process
  • Direct use of liquid medium - ready-to-use in sterile tubes or sterile bottle for individual portioning
  • Simple, visual screening by an indicator change from red to yellow

Order information

Product nameOrder numberDescriptionContent
NBB®-B-Am2.04706.782 Nutrient medium for qualitative detection
of indicator microorganisms
after sampling
with sterile swabs
Use of microbiological facility monitoring
9 x 250 ml
in tube
2.04706.646 Culture medium for qualitative detection
of indicator microorganisms
after sampling with sterile swabs
For microbiological facility monitoring
using an easy evaluation in glass tubes
with screw closure for direct application
20 x 10 ml
Smear swabs2.04725.444Sterile swabs,
cotton swabs on wooden sticks,
individually packaged,
without tubes
Smear samples
100 pc. (bag)

Basic information for NBB®-B-Am

pH valueIncubation T [°C]Duration (days)
5.75 +/- 0.1527Max. 3

Storage and shipping information for NBB®-B-Am

Storage temperature T [°C]Shelf life (months)

Packaging information for NBB®-B-Am

9 x 250 mlGlass bottles in cardboard box

Basic information for NBB®-B-Am in tubes

pH valueIncubation T [°C]Duration (days)
5.75 +/- 0.1527Max. 3

Storage and shipping information for NBB®-B-Am in tubes

Storage temperature T [°C]Shelf life (months)

Packaging information for NBB®-B-Am in tubes

20 x 10 mlGlass tubes with screw closure,
polystyrene box

Storage and shipping information for smear swabs

Storage temperature T [°C]Shelf life (months)
20-25 °CSee information on product

Packaging information for smear swabs

100 pc.Individually wrapped,
in bag

Further product recommendations


Please contact the following distributors or Döhler directly.

Country Name Address E-mail Phone
China Shanghai Sunny Biological Science
and Technology Co.Ltd
Room 1706, No.1619 Dalian road,
Hongkou District,
yhq@shennei.com +86 021 021 65190298 8016
Singapore Avantor SEA

18 Gul Drive
Singapore 629468

tharachaya.woraraktiwongs@avantorinc.com +66 95 5626963
Mongolia Monbiopharm Co. Ltd. Sukhbaatar District - 4 khoroo 1-69
First 40000
info@monbiopharm.mn +976 11 330234
Brazil Prozyn Indústria e Comércio Ltda Rua Dr. Paulo Leite de Oliveira, 199
São Paulo/SP
flavio.melo@prozyn.com.br +55 11 3732 0000
Brazil Merse R. Domingos Cazotti, 284
Jd. Santa Genebra - Campinas/SP 
valkiria@merse.com.br +55 193733 3800
USA Weber Scientific 2732 Kuser Road
Hamilton, NJ, 08691
swilson@weberscientific.com +1 609 249 1408
USA White Labs 9495 Candida St
San Diego, CA 92126

+1 858 527 7358

USA BSG 9650 East 40th Ave
Denver, CO 80238
rnelson@bsgcraftbrewing.com +1 303 574 8955
Canada ProSource Scientific 5370 Canotek Road Unit 11
Ontario K1J 9E7
duane@psscientific.com +1 866 939 9959
France Laboratories Humeau ZA Gesvrine - 4 rue Kepler BP 4125
44241 La Chapelle-sur-Erdre Cedex
m.minier@humeau.com +33 2 40 93 53 53
France Brewpark sas Rue des Colinettes
ZI Mardeuil, 51530 Mardeuil
michael.gelender@brewpark.com +33 3 26 55 82 89
Italy Sacco S.r.l. Via A. Manzoni 29/A
22071 Cadorago
p.berardinetti@saccosrl.it +39 031 8866 607 
Latvia SnapTest.LV SIA Valmieras iela 31B
Rīga, LV-1009
gints@snaptest.lv +371 26 53 47 45
United Kingdom Trafalgar Scientific Ltd 190 Waterside Rd, Leicester LE5 1QZ info@trafalgarscientific.co.uk +44 116 287 9460
Netherlands SBI Europe Kraaiondonk 44
5428 NZ Venhorst
The Netherlands
pvanrooij@sbi4beer.com +31 413 783 880
all European countries VWR International Hilpertstraße 20a
64295 Darmstadt
info.de@vwr.com +49 800 702 0007
all other countries Döhler GmbH Riedstraße 7-9
64295 Darmstadt
dmd@doehler.com +49 (0)6151 3060


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