Chromogenic Coliform Agar ISO

Differentiating chromogenic agar for the detection and bacterial count of E. coli and coliform bacteria in water samples according to ISO 9308-1

Product description

Chromogenic Coliform Agar (pH 6.8 ± 0.2) is a ready-to-use, selective and differentiating chromogenic agar for the detection and determination of bacteria count of Escherichia coli (E. coli) and coliform bacteria in water samples with minimal bacterial flora in the background, in accordance with ISO 9308-1 and the new German drinking water regulation.

 The combination of two chromogenic substrates allows the simultaneous detection of coliform bacteria and E. coli.  The chromogenic Salmon®-GAL substrate (6-chloro-3-indolyl-β-D-Galactopyranoside) is cleaved by the ß-D-Galactosidase, characteristic for coliforms, and results in a pink-red colouring of positive colonies. 

The cleavage of the chromogenic X-Glucuronide substrate (5-bromo-4-chloro-3-indoxyl-β-D-Glucuronide) by the ß-D-Glucuronidase, characteristic for E. coli, results in a blue colouring of positive colonies. The colonies turn dark blue violet as E. coli cleaves both Salmon®-GAL and X-Glucuronide. This makes it easy to distinguish them from the other coliforms which appear in a pink-red. The Tryptophan contained in the medium improves the indol reaction for the further confirmation of E. coli, thus increasing the detection reliability.

Order information

Product nameOrder numberDescriptionContent
Chromogenic Coliform Agar8.43330.244Chromogenic Coliform Agar
Application: Detection and bacterial count of
E. coli and coliform bacteria6 x 200 ml

Basic information

pH value Incubation T [°C]Duration (days)
6.8 +/-0.230-373

Storage and shipping information

Storage temperature T [°C]Shelf life (months)
2-8 12

Packaging information

6 x 200 mlGlass bottles in cardboard box

Please contact our distributors or Döhler directly.



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South Africa 385 04 83
AustraliaDirectus Australia96 Woolwich Rd
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NSW, 2110
Australia 2 9844 2100
ChinaShanghai Sunny Biological Science
and Technology Co.Ltd

Room 1706
No.1619 Dalian road
Hongkou District
Zip: 200086 021 55884753
+86 135 24671568
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168 Parnell Road
Auckland, 1052
New Zealand 9 309 7783
USAWeber Scientific2732 Kuser Road
Hamilton, NJ, 08691
USA 800 328 8378
rest of worldDöhler GmbH

Riedstraße 7-9
64295 Darmstadt
Germany (0)6151/306-0

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