For more than 30 years, Döhler has been offering innovative, ready-to-use culture media under its NBB® brand for the fast and reliable trace detection of beer spoiling bacteria, such as Lactobacillus, Pedicoccus, Pectinatus and Megasphaera, in all samples collected in breweries.
The newly developed and ready-to-use NBB®-PCR Broth now enables optimized enrichment of these beer spoiling bacteria for detection using molecular biological methods such as PCR or real-time PCR. Regardless of the PCR or real-time PCR system you use, NBB®-PCR broth is always compatible and designed for universal use.
The NBB®-PCR Broth offers you the following benefits:
In addition to conventional culture methods, molecular biological detection of beer spoiling bacteria is increasingly gaining importance. Besides fast and reliable approvals, the option of identifying existing microorganisms, down to their species is the driving force to establishthis procedure at breweries.
Efficient and selective enrichment of microorganisms continues to be necessary despite the increased sensitivity of the molecular biological methods. Because culture media consist of natural raw materials, the risk of DNA from beer spoiling bacteria is already being present in the medium during production. This DNA would generate “false positive” results for the analysed samples during detection.
Using a specially optimised production process, Döhler ensures that the ready-to-use NBB®-PCR Broth does not contain any DNA from beer spoiling bacteria.
This is continuously tested and certified by using a strict and complete quality control, from raw materials to end products.
With the optimised nutrient combination and the exclusion of ingredients inhibiting or interfering with PCR or real-time PCR, even enrichment of slowly growing microorganisms such as Lactobacillus lindneri or Pediococcus damnosus is possible within 48 to 72 hours. The ratio of growth substances and inhibitors in the medium ensures optimum selectivity, which has been confirmed in numerous analyses.
Strictly anaerobic beer spoiling bacteria such as Pectinatus and Megasphaera can also be detected in this period of time. The detection limit is also strongly influenced by the PCR or real-time PCR detection system used – i.e. purification, subsequent detection, as well as by the thermal cycler itself – rather than being solely dependent on the enrichment method.
The ready-to-use NBB®-PCR broth is suitable for detecting microorganism in all samples collected in breweries. Whether you need to detect beer spoiling bacteria in yeasts, such as propagation and harvesting yeasts, or in filterable and yeast-turbid beer, NBB®-PCR Broth can be used universally with all samples.
Detection performed using all PCR or real-time PCR systems available on the market has shown that NBB®-PCR Broth is a suitable pre-enrichment medium for all of these systems. NBB®-PCR can be used as an enrichment medium – regardless of the PCR or real-time PCR detection system you choose or have chosen.
NBB®-PCR Broth is ready-to-use and can be used immediately. Cumbersome and time-consuming weighing, dissolving, adjusting the pH and autoclaving is a thing of the past – and by using specific test strains, we have already taken care of the extensive quality control for you.
Please contact the following distributors or Döhler directly.
|China||Shanghai Sunny Biological Science |
and Technology Co.Ltd
|Room 1706, No.1619 Dalian road, |
|firstname.lastname@example.org||+86 021 021 65190298 8016|
|Singapore||Avantor SEA|| |
18 Gul Drive
|email@example.com||+66 95 5626963|
|Mongolia||Monbiopharm Co. Ltd.||Sukhbaatar District - 4 khoroo 1-69 |
|firstname.lastname@example.org||+976 11 330234|
|Brazil||Prozyn Indústria e Comércio Ltda||Rua Dr. Paulo Leite de Oliveira, 199 |
|email@example.com||+55 11 3732 0000|
|Brazil||Merse||R. Domingos Cazotti, 284 |
Jd. Santa Genebra - Campinas/SP
|firstname.lastname@example.org||+55 193733 3800|
|USA||Weber Scientific||2732 Kuser Road |
Hamilton, NJ, 08691
|email@example.com||+1 609 249 1408|
|USA||White Labs||9495 Candida St |
San Diego, CA 92126
+1 858 527 7358
|USA||BSG||9650 East 40th Ave |
Denver, CO 80238
|firstname.lastname@example.org||+1 303 574 8955|
|Canada||ProSource Scientific||5370 Canotek Road Unit 11 |
Ontario K1J 9E7
|email@example.com||+1 866 939 9959|
|France||Laboratories Humeau||ZA Gesvrine - 4 rue Kepler BP 4125 |
44241 La Chapelle-sur-Erdre Cedex
|firstname.lastname@example.org||+33 2 40 93 53 53|
|France||Brewpark sas||Rue des Colinettes |
ZI Mardeuil, 51530 Mardeuil
|email@example.com||+33 3 26 55 82 89|
|Italy||Sacco S.r.l.||Via A. Manzoni 29/A |
|firstname.lastname@example.org||+39 031 8866 607|
|Latvia||SnapTest.LV SIA||Valmieras iela 31B |
|email@example.com||+371 26 53 47 45|
|United Kingdom||Trafalgar Scientific Ltd||190 Waterside Rd, Leicester LE5 1QZfirstname.lastname@example.org||+44 116 287 9460|
|Netherlands||SBI Europe||Kraaiondonk 44 |
5428 NZ Venhorst
|email@example.com||+31 413 783 880|
|all European countries||VWR International||Hilpertstraße 20a |
|firstname.lastname@example.org||+49 800 702 0007|
|all other countries||Döhler GmbH||Riedstraße 7-9 |
|email@example.com||+49 (0)6151 3060|