NBB® Nutrient Media for Beer-Spoiling Bacteria

Innovative detection system for beer-spoiling bacteria

Beer spoilage microorganisms are mainly facultative or obligate anaerobic lactic acid bacteria that are alcohol and hop tolerant, e.g. Lactobacillus brevis, L. lindneri or Pediococci, etc. The spoilage microbes may already be present in raw materials such as water or company yeasts and in subsequent production path from brew house to bottling, from unfiltered to bottled beer. They can be introduced, for example, by inadequate industrial hygiene, contaminated raw materials, empty reusable containers, returned beer, biofilm formation in bottling, or even by humans.

The NBB® portfolio comprises solid and liquid media in order to provide the ideal detection solution for all samples throughout the entire brewing process. During the development of this medium around 40 years ago, the inventor, Prof. Werner Back prioritised safety, speed, selectivity and cost efficiency. The discovery of specific growth promoters ensures that even bacteria with very slow growth can be safely detected within a short period of time, while a balanced system of inhibitors suppresses the dominating yeast culture.

Since then, NBB has faced the constant challenge of being able to detect new bacteria such as Lactobacillus acetotolerans. Every NBB production batch is tested for functionality using a bacterial panel of select isolates. This is why renowned customers active in the brewing industry around the world place their trust in this high standard, extensive knowledge and expertise. Publications containing independent investigations and comparisons reinforce the product’s position as the market leader.

  • NBB®-B (broth) is a ready-to-use broth primarily intended for testing brewing yeast for beer-spoiling bacteria. A pH-based colour indicator shows possible contaminations early on by changing colour from red to yellow.
  • Ready-to-use NBB®-C (concentrated broth) is used to test large sample volumes of unfiltered and filtered beer to safely detect even the smallest numbers of bacteria.
  • NBB®-A (agar) is a ready-to-use agar medium for quantitative detection, primarily of membrane filtered beer. A pH-based colour indicator shows possible contaminations early on by changing colour from red to yellow.
  • NBB®-PCR Broth is a ready-to-use liquid enrichment specifically for subsequent PCR analysis, see link.
  • NBB®-B-Am is used in hygiene monitoring, see link.

Benefits of product

Application benefits

- Ready-to-use nutrient media - Reference medium in EBC and MEBAK
- Functional test with practice isolates - Rapid growth through special growth promoters
- Reliable - Formats for all occurring sample matrices

Order information


Artikel Nr. 


NBB®-B (broth)



9 x 250 ml in glass bottles

20 x 10 ml in glass tubes

NBB®-C (concentrate) 204.711.782 9 x 250 ml in glass bottles
NBB®-A (agar) 204.709.782 9 x 250 ml in glass bottles

NBB®-P (powder)

(for the production of NBB®-A or -B)

204.716.462 1 x 300 g in aluminium bag
NBB®-PCR (Broth) 785.420.782 9 x 250 ml in glass bottles
NBB®-B-Am (Broth)



9 x 250 ml in glass bottles

20 x 10 ml in glass tubes

Further product recommendations

Please contact the following distributors or Döhler directly.

Country Name Adress Email Phone
China Shanghai Sunny Biological Science
and Technology Co.Ltd
Room 1706, No.1619 Dalian road,
Hongkou District,
yhq@shennei.com +86 021 65190298 8016

Avantor SEA

18 Gul Drive
Singapore 629468
tharachaya.woraraktiwongs@avantorinc.com +66 95 5626963
Mongolia Monbiopharm Co. Ltd. Sukhbaatar District - 4 khoroo 1-69
First 40000
info@monbiopharm.mn +976 11 330234
Brazil Prozyn Indústria e Comércio Ltda Rua Dr. Paulo Leite de Oliveira, 199
São Paulo/SP
flavio.melo@prozyn.com.br +55 11 3732 0000
Brazil Merse R. Domingos Cazotti, 284
Jd. Santa Genebra - Campinas/SP 
valkiria@merse.com.br +55 193733 3800
USA Weber Scientific 2732 Kuser Road
Hamilton, NJ, 08691
swilson@weberscientific.com +1 609 249 1408
USA White Labs 9495 Candida St
San Diego, CA 92126
nparker@whitelabs.com +1 858 527 7358
USA BSG 9650 East 40th Ave
Denver, CO 80238
rnelson@bsgcraftbrewing.com +1 303 574 8955
Canada ProSource Scientific 5370 Canotek Road Unit 11
Ontario K1J 9E7
duane@psscientific.com +1 866 939 9959
France Laboratories Humeau ZA Gesvrine - 4 rue Kepler BP 4125
44241 La Chapelle-sur-Erdre Cedex
m.minier@humeau.com +33 2 40 93 53 53
France Brewpark sas Rue des Colinettes
ZI Mardeuil, 51530 Mardeuil
michael.gelender@brewpark.com +33 3 26 55 82 89
Italy Sacco S.r.l. Via A. Manzoni 29/A
22071 Cadorago
p.berardinetti@saccosrl.it +39 031 8866607
Latvia SnapTest.LV SIA Valmieras iela 31B
Rīga, LV-1009
gints@snaptest.lv +371 26 53 47 45
United Kingdom Trafalgar Scientific Ltd 190 Waterside Rd, Leicester LE5 1QZ asmith@trafalgarscientific.co.uk  +44 116 287 9460
Netherlands SBI Europe Kraaiondonk 44
5428 NZ Venhorst
The Netherlands
pvanrooij@sbi4beer.com +31 413 783 880
all European countries VWR International Hilpertstraße 20a
64295 Darmstadt
info.de@vwr.com +49 800 702 00 07
all other countries Döhler GmbH Riedstraße 7-9
64295 Darmstadt
dmd@doehler.com +49 (0)6151 3060

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