BAT Culture Media and Guaiacol Detection Kit

Detection of Alicyclobacilli spp. and Alicyclobacilli which form guaiacol 

Alicyclobacilli are aerobic, acidophilic and thermophilic bacteria that can grow in highly acidic beverages such as in fruit juices, but also in the raw materials for these, such as fruit juice concentrates or sugar syrup. With their ability to form spores, they can survive the pasteurisation process and continue to grow vegetatively under optimal conditions. According to scientific findings, only 3 of the approximately 40 known alicyclobacilli have the potential to produce the guaiacol which spoils beverages with its strong off-flavour (intense chemical smell).

The detection method takes place in 2 steps. First, the possible contamination is enriched and isolated via cultivation with our ready-to-use nutrient media BAT Broth and Agar. In the second step, the biochemical Guaiacol Detection Kit is used to analyse the isolated alicyclobacillus bacteria for its ability to form guaiacol.

The BAT Culture Media and the Guaiacol Detection Kit are described in the IFU (International Fruit and Vegetable Juice Association) Method No.12 "Method on the Detection and Enumeration of Spore-forming Thermo-Acidophilic Spoilage bacteria“

Benefits of productApplication benefits
- Ready for immediate use- Standardised detection as per IFU Method No. 12
- Functional testing with special test bacteria- Easy handling
- High detection reliability- No additional laboratory equipment required

Order information

ProductArt. no. Packaging
BAT-A204.719.7829 x 250 ml in glass bottles
BAT-B204.717.7829 x 250 ml in glass bottles
Guaiacol Detection Kit204.737.991

40 x 2ml BAT-IB in glass tubes

plus buffer solution, guaiacol standard solution, lyophilised enzyme, hydrogen peroxide

Further product recommendations

Please contact the following distributors or Döhler directly.

ChinaShanghai Sunny Biological Science
and Technology Co.Ltd
Room 1706, No.1619 Dalian road,
Hongkou District,
Shanghai 021 65190298 8016

Avantor SEA

18 Gul Drive
Singapore 629468 95 5626963
MongoliaMonbiopharm Co. Ltd.Sukhbaatar District - 4 khoroo 1-69
First 40000
Ulanbaatar 11 330234
BrazilProzyn Indústria e Comércio LtdaRua Dr. Paulo Leite de Oliveira, 199
São Paulo/SP 11 3732 0000
BrazilMerseR. Domingos Cazotti, 284
Jd. Santa Genebra - Campinas/SP 193733 3800
USAWeber Scientific2732 Kuser Road
Hamilton, NJ, 08691 609 249 1408
USAWhite Labs9495 Candida St
San Diego, CA 92126 858 527 7358
USABSG9650 East 40th Ave
Denver, CO 80238 303 574 8955
CanadaProSource Scientific5370 Canotek Road Unit 11
Ontario K1J 9E7 866 939 9959
FranceLaboratories HumeauZA Gesvrine - 4 rue Kepler BP 4125
44241 La Chapelle-sur-Erdre Cedex 2 40 93 53 53
FranceBrewpark sasRue des Colinettes
ZI Mardeuil, 51530 Mardeuil 3 26 55 82 89
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22071 Cadorago 031 8866607
LatviaSnapTest.LV SIAValmieras iela 31B
Rīga, LV-1009 26 53 47 45
United KingdomTrafalgar Scientific Ltd190 Waterside Rd, Leicester LE5 +44 116 287 9460
NetherlandsSBI EuropeKraaiondonk 44
5428 NZ Venhorst
The Netherlands 413 783 880
all European countriesVWR InternationalHilpertstraße 20a
64295 Darmstadt
Germany 800 702 00 07
all other countriesDöhler GmbHRiedstraße 7-9
64295 Darmstadt
Germany (0)6151 3060

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