NBB®

NBB® ready-to-use media for the qualitative and quantitative detection

of beverage-spoiling microorganisms in all samples arising in breweries and for the wine market.

NBB® culture media are suitable for detection in: 

  • yeast samples
  • unfiltered beers containing yeast
  • filtered beers
  • wine
  • new wine
  • water and rinsing water
  • airborne bacteria collection samples
  • swab samples for hygiene monitoring

Product description

Under its NBB® brand, Döhler offers innovative culture media for the fast and reliable detection of beverage spoiling microorganisms, such as Lactobacilli, Pectinatus and Megasphaera, in all samples that arise in breweries, as well as for the wine industry.

The desire for reliable and easy-to-use cultural detection methods has driven the development of the NBB® culture media, which has been ongoing for 30 years now. Clear and fast detection, a reliable result – even for a very low level of contamination in a large sample volume – and easy application for efficient laboratory work are the key driving forces behind these developments.

That is why renowned customers active in the beverage industry around the world place their trust in this high standard, extensive knowledge and expertise. Publications containing independent investigations and comparisons reinforce the product’s position as the market leader.

With their special growth promotion components and nutrients optimally tailored to beer and wine spoiling microorganisms, NBB® culture media offer a multitude of advantages. Döhler exclusively uses raw materials of non-animal origin, thus eliminating the risk of animal materials entering the beverage quality control process. A series of comprehensive functional tests examine the quality of the NBB culture media with a special panel of test strains, which were all isolated in breweries.

The NBB® portfolio includes the following products:

NBB®-A, Agar

Solid agar medium for pouring agar plates after simple liquefaction 

  • Reduced preparation time by direct liquefaction in the bottle
  • Can be used for membrane filtration, swab and pour plate method as well as for airborne microorganism collection
  • Simple, visual screening by means of an indicator change from red to yellow

NBB®-B, Broth

Liquid culture media with indicator dye for detection of beer spoiling microorganisms in yeast samples or as an enrichment medium

  • Direct use of liquid medium
  • Simple, visual screening by an indicator change from red to yellow

NBB®-C, Concentrated Broth

Liquid culture medium with enhanced selectivity through the addition of yeast-cloudy beer samples.

  • Simply added to the beer sample for analysis
  • Final microscopic inspection, no indicator verification

NBB®-B-AM, Broth

Liquid culture medium for detection of indicator bacteria from biofilms

  • Simple hygiene monitoring for weak point analyses and trending
  • Sampling by means of smear swabs at critical points throughout the production process
  • Direct use of liquid medium - ready-to-use in sterile tubes or sterile bottle for individual portioning
  • Simple, visual screening by means of an indicator change from red to yellow

NBB® culture media offer you the following user benefits:

Flexible formats for immediate use  –  ready-to-use culture media can be used immediately; there is no need for complex weighing of individual components, handling poisonous substances, forming solutions or autoclaving

There is a suitable, optimised NBB® culture medium for every sample to be analysed in the brewing process!

NBB® – Method

Sample typeProductFormatMethod
Yeast samples
Selected, harvested and brewing yeast
Yeast sediments
NBB®-B

Broth in bottle

0.5-1 ml of sample + 10-20 ml of NBB®-B
Broth in tube
Yeast-cloudy beers
Green beer
Unfiltrate, wheat beer
NBB®-CConcentrated broth95% sample + 5% NBB®-C
Clear beers
Membrane filtered samples
NBB®-AAgarFiltration of 50-200 ml of sample
Water, rinsing water
Membrane filtered samples
NBB®-AAgarFiltration of 50-200 ml of sample
Ambient air
Airborne microorganism collection on agar plates
NBB®-AAgarDirect collection
Surfaces in filling plants
Hygiene monitoring using smear swabs
NBB®-B-AmBroth 1 smear swab in 10 ml of NBB®-BAM
NBB®-PPowderFor the production of NBB®-A and NBB®-B
Laboratory accessoriesSmear swab, without tube
  • Fast results – optimised nutrient combinations for fast growth of spoiling microorganisms 
  • Simple screening – Simple and reliable visual control through a clear change in the indicator colour from red to yellow, for easy quantitative and qualitative analysis  in almost any NBB® culture media
  • Increased selectivity – Inhibition of the harmless accompanying flora and culture yeast necessary for the brewing process for optimised detection of beverage-spoiling microorganisms

Order information

Product nameOrder numberDescriptionContent
NBB®-A2.04709.782Selective agar for the isolation,
cultivation and quantitative
detection in filtered beer
Application:
Basic nutrient media with indicator dye
for membrane filtration, casting plate process
9 x 250 ml
NBB®-B2.04710.782Selective liquid medium
for fast trace detection
in yeast culture
Application:
Sample enrichment with integrated
indicator for simple assessment
9 x 250 ml
NBB®-B
in tube
2.04723.646 Selective liquid medium
for fast trace detection
in yeast culture
Application:
Sample enrichment with integrated
indicator for simple assessment in
glass tubes with screw closure
for immediate application
20 x 10 ml
NBB®-C2.04711.782Concentrated, selective liquid medium
for fast trace detection
in unfiltered beer
Application:
Sample enrichment for cloudy and non-filterable
samples, use of large sample volumes
9 x 250 ml
NBB®-B-Am2.04706.782Nutrient medium for qualitative detection
of indicator microorganisms after sampling
with sterile swabs
Application:
Use in microbiological facility monitoring
9 x 250 ml
NBB®-B-Am
in tube
2.04706.646Culture medium for qualitative detection
of indicator microorganisms after sampling
with sterile swabs
Application:
For microbiological facility monitoring
using an easy evaluation in glass tubes
with screw closure for direct application
20 x 10 ml
NBB®-P2.04716.462Powder for the production
of NBB® Agar and NBB® Broth
Selectivity adjustment through
use of own beer
300 g (bag)
Smear swabs2.04725.444Sterile cotton swabs on wooden sticks,
individually packaged,
without tubes
Application:
Smear samples
100 pc. (bag)

Basic information for NBB®-A

pH valueIncubation T [°C]Duration (days)
5.8 +/- 0.225-285

Storage and shipping information for NBB®-A

Storage temperature T [°C]Shelf life (months)
4-836

Packaging information for NBB®-A

QuantityPackaging
9 x 250 mlGlass bottles in cardboard box

Basic information for NBB®-B

pH valueIncubation T [°C]Duration (days)
5,8 +/- 0.225-285

Storage and shipping information for NBB®-B

Storage temperature T [°C]Shelf life (months)
4-836

Packaging information for NBB®-B

QuantityPackaging
9 x 250 mlGlass bottles in cardboard box

Basic information for NBB®-B in tubes

pH valueIncubation T [°C]Duration (days)
5.8 +/- 0.225-285

Storage and shipping information for NBB®-B in tubes

Storage temperature T [°C]Shelf life (months)
4-836

Packaging information for NBB®-B in tubes

QuantityPackaging
20 x 10 mlGlass tubes with screw closure,
polystyrene box

Basic information for NBB®-C

pH valueIncubation T [°C]Duration (days)
6.4 +/- 0.225-28Beer samples: 7-14;
yeast samples: 5

Storage and shipping information for NBB®-C

Storage temperature T [°C]Shelf life (months)
4-836

Packaging information for NBB®-C

QuantityPackaging
9 x 250 mlGlass bottles in cardboard box

Basic information for NBB®-B-Am

pH valueIncubation T [°C]Duration (days)
5.8 +/- 0.227Max. 3

Storage and shipping information for NBB®-B-Am

Storage temperature T [°C]Shelf life (months)
4-836

Packaging information for NBB®-B-Am

QuantityPackaging
9 x 250 mlGlass bottles in cardboard box

Basic information for NBB®-B-Am in tubes

pH valueIncubation T [°C]Duration (days)
5.8 +/- 0.227Max. 3

Storage and shipping information for NBB®-B-Am in tubes

Storage temperature T [°C]Shelf life (months)
4-836

Packaging information for NBB®-B-Am in tubes

QuantityPackaging
20 x 10 mlGlass tubes with screw closure,
polystyrene box

Basic information for NBB®-P

pH value (+/- 0.2)Incubation T [°C]Duration (days)
Adjustment in line with specification
at customer premises,
pH values between
5.6 and 5.9
25-285

Storage and shipping information for NBB®-P

Storage temperature T [°C]Shelf life (months)
4-818

Packaging information for NBB®-P

QuantityPackaging
300gAluminium bag

Storage and shipping information for smear swabs

Storage temperature T [°C]Shelf life (months)
20-25°CSee information on product

Packaging information for smear swabs

QuantityPackaging
100 pc.Individually wrapped,
in bag

Please contact our distributors or Döhler directly.

LandNameAdressEmailPhone

Africa*

Dematech Pty Ltd.13 Michigan Street
Airport Industria
Capetown 7490
South Africa
info@dematechsa.com+27-(021) 385 04 83
AustraliaDirectus Australia96 Woolwich Rd
Hunters Hill
NSW, 2110
Australia
sales@directus.com.au+61 2 9844 2100
ChinaShanghai Sunny Biological Science
and Technology Co.Ltd

Room 1706
No.1619 Dalian road
Hongkou District
Shanghai
China
Zip: 200086

yanhqx@sina.com+86 021 55884753
+86 135 24671568
New ZealandDirectus New ZealandSuite 2, Level 1, Heards
168 Parnell Road
Auckland, 1052
New Zealand
sales@directus.co.nz+64 9 309 7783
USAWeber Scientific2732 Kuser Road
Hamilton, NJ, 08691
USA
info@weberscientific.com+1 800 328 8378
rest of worldDöhler GmbH

Riedstraße 7-9
64295 Darmstadt
Germany

dmd@doehler.com+49 (0)6151/306-0

*excl.: Libya, Sudan, Morocco, Mauritania, Western Sahara, Tunisia, Egypt, Algeria

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