DMD® Screen and Ident Kits

Efficient and reliable detection of beer spoiling bacteria in just an hour

DMD® Screen and Ident Kits

The DMD Screen & Ident Kits  are suitable for PCR and subsequent visual detection on a test strip from

  • yeast samples
  • yeast-turbid beers
  • filtered beers
  • water and rinsing water
  • airborne microorganism sampling
  • swab samples for hygiene monitoring

Product description

Simple, fast, reliable – Döhler's DMD® Screen & Ident Kits are transforming microbiological quality control in breweries. In addition to conventional culture methods, molecular biological detection of beer spoiling bacteria is gaining importance. In the past, molecular analysis using real-time PCR was very cumbersome and cost-intensive, and could only be conducted by trained personnel.

With the DMD® Screen & Ident Kits, Döhler provides breweries with a simple, cost-effective and fast method of detecting beer spoiling bacteria such as Lactobacillus, Pediococcus, Pectinatus and Megasphaera in all samples collected in breweries.

The kits allow bacteria in an enriched beer sample to be detected and identified in just an hour. This is achieved by combining molecular-biological PCR detection with simple visual analysis on a test strip. This combination reduces the time needed for cultural detection – usually around five days or more – to just two to three days, including pre-enrichment. The system's ease of use makes it especially attractive: no specific molecular-biological expertise is needed.

Comprehensive detection portfolio with DMD® Screen and Ident Kits

The constantly-expanding portfolio for the detection of beer spoiling bacteria comprises kits for the screening of Lactobacillus and Pediococcus, the  DMD® Lactobacillus Pediococcus Screen Kit and of Pectinatus and Megasphaera, the DMD® Pectinatus Megasphaera Screen Kit. After positive screening for Lactobacillus/Pediococcus, the DMD® Lactobacillus brevis Ident Kit can be used to identify Lactobacillus brevis as beer spoiling bacteria. This bacteria is the cause of the beer spoiling in around 80 per cent of cases. 

In addition, the DMD® Hop Resistance Screen Kit provides fast detection of hop-resistant microorganisms. Hop resistance is caused by horA and horC genes, both of which can be detected with the DMD® Hop Resistance Screen Kit. Just one of these genes is sufficient to provide the potential to spoil beer. This unique tool allows unknown bacteria to be tested for their potential to spoil beer.

All DMD® Screen and Ident Kits must be combined with the DMD® Universal Detection Module allowing the detection on a test strip.

The DMD® Screen & Ident Kits offers you the following user benefits:

  • Innovative technology with no need for expert knowledge or expensive equipment
  • Easy to use with the reagents included in the kit
  • Outstanding reliability through visual analysis and integrated amplification control
  • Comprehensive solution with optimum enrichment

Innovative technology with no need for expert knowledge or expensive equipment

The innovative DMD® Screen & Ident Kits combine the high sensitivity of PCR (polymerase chain reaction) with the simplicity of lateral flow based detection. The positive or negative result is easy to read from coloured bands on a test strip, with no need for expert knowledge or expensive equipment. The cost-efficient investment in a thermal cylcer allows the direct access to the technology.

Easy to use with the reagents included in the kit

The reagents provided and the illustrated manual make application very easy.First, the enriched sample is mixed with the prepared reaction mix and heated in a PCR thermal cycler. This releases the genetic material of any microorganisms present, which can then be amplified. There is no need for cumbersome DNA purification with special protocols, so a small quantity can be applied to a test strip after a PCR programme of just 50 minutes. Once placed  in an assay buffer, the test strip is ready for analysis after another five minutes.

Testing can be done from a liquid enrichment in a culture medium optimised for PCR or through direct analysis of individual colonies on solid media.

All DMD® Screen and Ident Kits contain all the reagents needed for reliable and efficient detection. Besides the PCR-Polymerase Master Mix (2x) and the specific PCR Primer Mix (2x), they also contain a positive control and PCR-grade water for dilution and as a negative control.

DMD® Screen and Ident Kit

All DMD® Screen and Ident Kits need to be combined with the DMD® Universal Detection Module. The kit contains the assay buffer and test strips needed to make the result visible.

DMD® Universal Detection Module

Outstanding reliability through visual analysis and integrated amplification control

The analysis results on the DMD® Universal Detection Module test strip. The red arrow shows the direction of the assay buffer travel.

  • Strips 1 and 2 show clear positive results.
  • Strip 3 shows a negative result.
  • Strip 4 shows potential inhibition of the PCR – the test should be repeated.
  • Strip 5 cannot be analysed.


Results are displayed by easy-to-read visible bands, similar to a pregnancy test. The products amplified in the PCR are applied to the DMD® Universal Detection Module test strips. The test strips are placed in the assay buffer included in the kit and are analysed visually after five minutes. The bands that appear provide a clear, easy-to-interpret result.

The use of an internal PCR amplification control is very important for this clear result. It shows whether a negative result is clearly negative because no DNA from the relevant microorganism is present or whether the PCR has been inhibited. This makes the analysis reliable and prevents false positive and false negative results, which can result in cumbersome investigation or even disposal of a flawless end product.

Comprehensive solution with optimum enrichment

Beer samples need to be enriched before detection with the DMD® Screen & Ident Kits. The ready-to-use NBB®-PCR Broth is ideal for this. Döhler is the only company to provide a comprehensive solution for PCR detection of beer spoiling bacteria and subsequent detection on a test strip.

The optimised composition of the medium promotes fast growth of beer spoiling bacteria, enabling enrichment and detection within just 48 to 72 hours, even of slow-growing microorganisms like Lactobacillus lindneri or Pediococcus damnosus, for example. This offers an unique advantage: a special production process makes NBB®-PCR Broth free from DNA of beer spoiling bacteria and PCR inhibitors. As a result, false positive and false negative results, which can have expensive consequences for breweries, are avoided.

NBB®-PCR Broth

Product nameOrder numberDescriptionReactions per KitContent
DMD® Lactobacillus
Pediococcus
Screen Kit
8.20576.244Reagents for
PCR amplification of
beer spoiling
bacteria:
Lactobacillus ssp. and
Pediococcus ssp.
48
  • PCR Primer Mix  (2x) –  2x 280 µl
  • PCR-Polymerase Mix (2x) –  2 x 300 µl
  • LP & HR positive control – 1 x 250 µl
  • PCR Wasser – 1 x 1000 µl
DMD® Pectinatus
Megasphaera
Screen Kit
8.20604.244Reagents for
PCR amplification of
beer spoiling
bacteria:
Hop resistance genes
horA and horC
48

  • PCR Primer Mix  (2x) –  2x 280 µl
  • PCR-Polymerase Mix (2x) –  2 x 300 µl
  • LP & HR positive control – 1 x 250 µl
  • PCR Wasser – 1 x 1000 µl
DMD® Hop
Resistance Kit
8.20607.244Reagents for
PCR amplification of
beer spoiling
bacteria:
Hop resistance genes
horA and horC

48
  • PCR Primer Mix  (2x) –  2x 280 µl
  • PCR-Polymerase Mix (2x) –  2 x 300 µl
  • LP & HR positive control – 1 x 250 µl
  • PCR Wasser – 1 x 1000 µl
DMD® Lactobazillus
brevis Ident Kit
8.20608.244Reagents for
PCR amplification of
beer spoiling
bacteria:
Lactobacillus brevis
24
  • PCR Primer Mix  (2x) –  2x 280 µl
  • PCR-Polymerase Mix (2x) –  2 x 300 µl
  • LP & HR positive control – 1 x 250 µl
  • PCR Wasser – 1 x 1000 µl
DMD® Universal Detection
Module
8.20610.244Reagents for
detection of the
PCR products based
on lateral flow
48
  • Assay-Buffer – 5 ml
  • 48 test strips
Product nameStorage temperature T [°C]Shelf life (months)
DMD® Lactobacillus Pediococcus Screen Kit-2012
DMD® Pectinatus Megasphaera Screen Kit-2012
DMD® Hop Resistance Kit-2012
DMD® Lactobazillus brevis Ident Kit-2012
DMD® Universal Detection Module+4-+824
Product namePackaging
DMD® Lactobacillus Pediococcus Screen KitZiplock bag with plastic box 
DMD® Pectinatus Megasphaera Screen KitZiplock bag with plastic box 
DMD® Hop Resistance KitZiplock bag with plastic box 
DMD® Lactobazillus brevis Ident KitZiplock bag with plastic box 
DMD® Universal Detection ModuleCardboard box

Please contact our distributors or Döhler directly.

LandNameAdressEmailPhone

Africa*

Dematech Pty Ltd.13 Michigan Street
Airport Industria
Capetown 7490
South Africa
info@dematechsa.com+27-(021) 385 04 83
AustraliaDirectus Australia96 Woolwich Rd
Hunters Hill
NSW, 2110
Australia
sales@directus.com.au+61 2 9844 2100
ChinaShanghai Sunny Biological Science
and Technology Co.Ltd

Room 1706
No.1619 Dalian road
Hongkou District
Shanghai
China
Zip: 200086

yanhqx@sina.com+86 021 55884753
+86 135 24671568
New ZealandDirectus New ZealandSuite 2, Level 1, Heards
168 Parnell Road
Auckland, 1052
New Zealand
sales@directus.co.nz+64 9 309 7783
USAWeber Scientific2732 Kuser Road
Hamilton, NJ, 08691
USA
info@weberscientific.com+1 800 328 8378
rest of worldDöhler GmbH

Riedstraße 7-9
64295 Darmstadt
Germany

dmd@doehler.com+49 (0)6151/306-0

*excl.: Libya, Sudan, Morocco, Mauritania, Western Sahara, Tunisia, Egypt, Algeria

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